mruby2 pcdna3 mruby2 Search Results


mruby2  (Addgene inc)
93
Addgene inc mruby2
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+pcdna3+mruby2/pcDNA3-mRuby2+(Plasmid+%2340260)/pmc06119192-228-11-21
Average 93 stars, based on 1 article reviews
mruby2 - by Bioz Stars, 2026-09
93/100 stars
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pcdna3 1 clover mruby2  (Addgene inc)
93
Addgene inc pcdna3 1 clover mruby2
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Pcdna3 1 Clover Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+pcdna3+mruby2/pcDNA3%2E1-Clover-mRuby2+(Plasmid+%2349089)/pmc09636422-46-4-17
Average 93 stars, based on 1 article reviews
pcdna3 1 clover mruby2 - by Bioz Stars, 2026-09
93/100 stars
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par sensor pcdna3 pbz mruby2  (Addgene inc)
93
Addgene inc par sensor pcdna3 pbz mruby2
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Par Sensor Pcdna3 Pbz Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+pcdna3+mruby2/pcDNA3-PBZ-mRuby2+(Plasmid+%23110650)/pm37823600-247-17-20
Average 93 stars, based on 1 article reviews
par sensor pcdna3 pbz mruby2 - by Bioz Stars, 2026-09
93/100 stars
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addgene plasmid  (Addgene inc)
90
Addgene inc addgene plasmid
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+pcdna3+mruby2/pcDNA3%2E1-Clover-Prx2-mRuby2+(Plasmid+%23117906)/pm34678160-458-154-154
Average 90 stars, based on 1 article reviews
addgene plasmid - by Bioz Stars, 2026-09
90/100 stars
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plasmid pcdna3  (Addgene inc)
94
Addgene inc plasmid pcdna3
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Plasmid Pcdna3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+pcdna3+mruby2/pcDNA3+mRuby2+LIC+cloning+vector+(6H)+(Plasmid+%2386965)/pm39969205-272-5-16
Average 94 stars, based on 1 article reviews
plasmid pcdna3 - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both mRuby2-UCHD and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )

Journal: Nature Communications

Article Title: Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

doi: 10.1038/s41467-018-05851-9

Figure Lengend Snippet: Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both mRuby2-UCHD and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )

Article Snippet: The EGFP sequence of pT24xnrUAS:EGFP-UCHD was replaced by the sequence of mRuby2 (amplified from pcDNA3-mRuby2 was a gift from Michael Lin; Addgene plasmid #40260) or by the sequence of mClav2 (amplified from pmClavGR2-NT; Allele Biotechnology) to generate the final plasmids pT24xnrUAS:mRuby2-UCHD and pT24xnrUAS:mClav2-UCHD respectively.

Techniques: Expressing, Injection, Plasmid Preparation